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Product Info Summary
| SKU: | A00716-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SNAIL/SNAI1 Antibody Picoband®
SKU/Catalog Number
A00716-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNAIL/SNAI1 Antibody Picoband® catalog # A00716-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SNAIL/SNAI1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00716-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SNAIL/SNAI1 recombinant protein (Position: M1-K170).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00716-2 is reactive to SNAI1 in Human, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.1 kDa
Background of SNAI1
The Drosophila embryonic protein SNAI1, commonly known as Snail, is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. And it is located in 16q24.3. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. It is studied that SNAIL gene may show a role in recurrence of breast cancer by downregulating E-cadherin and inducing anepithelial to mesenchymal transition.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00716-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A431 whole cell, human HepG2 whole cell, human A549 whole cell, human PC-3 whole cell, human K562 whole cell, human A431 whole cell, human SW620 whole cell, human Raji whole cell, rat RH35 whole cell, mouse liver tissue, mouse lung tissue, mouse heart tissue, mouse HEPA1-6 whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of SNAIL/SNAI1 using anti-SNAIL/SNAI1 antibody (A00716-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human SW620 whole cell lysates,
Lane 8: human Raji whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAIL/SNAI1 antigen affinity purified polyclonal antibody (Catalog # A00716-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAIL/SNAI1 at approximately 29 kDa. The expected band size for SNAIL/SNAI1 is at 29 kDa.
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Flow Cytometry analysis of 293T cells using anti-SNAIL/SNAI1 antibody (A00716-2).
Click image to see more details
Western blot analysis of SNAIL/SNAI1 using anti-SNAIL/SNAI1 antibody (A00716-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat RH35 whole cell lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse HEPA1-6 whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAIL/SNAI1 antigen affinity purified polyclonal antibody (Catalog # A00716-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAIL/SNAI1 at approximately 29 kDa. The expected band size for SNAIL/SNAI1 is at 29 kDa.
Specific Publications For Anti-SNAIL/SNAI1 Antibody Picoband® (A00716-2)
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