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Product Info Summary
| SKU: | A13669 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SNX25 Antibody Picoband®
SKU/Catalog Number
A13669
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNX25 Antibody Picoband® catalog # A13669. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SNX25 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13669)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SNX25 recombinant protein (Position: H48-K584). Human SNX25 shares 86.4% amino acid (aa) sequence identity with mouse SNX25.
Reactive Species
A13669 is reactive to SNX25 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
97.9 kDa
Background of SNX25
SNX25 (sorting nexin 25), also known as SBBI31 or MSTP043, is an 840 amino acid protein suggested to function in several stages of intracellular trafficking. A member of the sorting nexin family, SNX25 contains one PX (phox homology) domain, an RGS domain and one PXA domain. May be involved in several stages of intracellular trafficking.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13669 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 0.25-0.5 μg/ml | Human, Mouse, Rat |
| Flow Cytometry (FC) | 1-3 μg/1x106 cells | Human |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of SNX25 using anti-SNX25 antibody (A13669).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX25 antigen affinity purified polyclonal antibody (Catalog # A13669) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX25 at approximately 100 kDa. The expected band size for SNX25 is at 98 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-SNX25 antibody (A13669).
Overlay histogram showing HepG2 cells stained with A13669 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX25 Antibody (A13669, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-SNX25 Antibody Picoband® (A13669)
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