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- Table of Contents
SOX9 antibody (M00177) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Immunofluorescent analysis of SW480 cells, using SOX9 Antibody .
Designing a Western blot for SOX9? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the SOX9 Western Blot GuideBundle Save
Anti-SOX9 Rabbit Monoclonal Antibody
SOX9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
SOX9 antibody was positively detected by WB in:
human Hela whole cell, human CACO-2 whole cell, human SW620 whole cell, rat PC-12 whole cell, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SOX9 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human liver cancer tissue, human prostate cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SOX9 antibody was positively detected by ICC/IF in:
SW480 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-SOX9 Rabbit Monoclonal Antibody
View all SOX9 Antibodies
View all SOX9 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DAO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOX9
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
56.1 kDa
Cite This Product
Anti-SOX9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00177)
Protocols
Western Blot protocol for SOX9 antibody
Immunohistochemistry (IHC) protocol for SOX9 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SOX9 antibody
Publications
Application:
Species:
Cyanidin-3-O-glucoside chloride inhibits cartilage degeneration and inflammation in TMJOA via YAP/NF-κB signaling pathway
Published: 2026-02-17
Journal: Food & Function
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 41725564
LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts
Published: 2024-10-15
Journal: Molecular Medicine Reports
Application: IF
Impact Factor: 3.40
Species: Human
PMID: 39422037
AHR regulates liver enlargement and regeneration through the YAP signaling pathway
Published: 2024-09-02
Journal: Heliyon
Application: IHC
Impact Factor: 3.40
Species: Mouse
PMID: 39296106
Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration
Published: 2023-09-22
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: IF
Impact Factor: 5.40
Species: Rat
PMID: 37772372
Inhibition of p38MAPK signal pathway alleviates radiation-induced testicular damage through improving spermatogenesis
Published: 2023-08-14
Journal: BRITISH JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 7.30
Species: Mouse
PMID: 37580308
Icariin increases chondrocyte vitality by promoting hypoxia-inducible factor-1α expression and anaerobic glycolysis
Published: 2019-12-26
Journal: KNEE
Application: WB
Impact Factor: 1.76
Species: Mouse
PMID: 31883860
Reelin regulates male mouse reproductive capacity via the sertoli cells
Published: 2018-10-18
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: IF
Impact Factor: 2.96
Species: Mouse
PMID: 30335884
Identification and differentiation therapy strategy of pterygium in vitro
Published: 2018-08-15
Journal: American Journal of Translational Research
Application: IF
Impact Factor: 3.06
Species: Mouse
PMID: 30210698
RETRACTED: Functional quantum dot-siRNA nanoplexes to regulate chondrogenic differentiation of mesenchymal stem cells
Published: 2016-09-09
Journal: Acta Biomaterialia
Application: IHC
Impact Factor: 6.01
Species: Rat
PMID: 27615736
Review
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1 Review For Anti-SOX9 Rabbit Monoclonal Antibody
Anti-SOX9 Antibody (M00177) produced distinct bands and notable expression differences across groups in WB of mouse brown adipose tissue, with GAPDH as loading control.
| SKU | M00177 |
|---|---|
| Application | Western Blot |
| Sample | Mouse adipocytes |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | 5% non-fat milk |
| Primary Antibody | SOX9 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:2000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ABBKINE |
| Results Summary | The image displays Western blot results for the target protein SOX9 and the internal control GAPDH across different experimental groups. The target bands are clear and distinct, showing significant differential expression between the experimental groups; the results are satisfactory. |
Jiaqi Xin, Capital Medical University
Verified customer
Submitted 2026-11-05
Product Promise
We promise Anti-SOX9 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00177
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026