Product Info Summary
SKU: | M00177 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SOX9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00177
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SOX9 Rabbit Monoclonal Antibody catalog # M00177. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00177)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DAO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOX9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00177 is reactive to SOX9 in Human, Mouse, Rat
Applications
M00177 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
56.137kDa
Background of SOX9
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SOX9 using anti-SOX9 antibody (M00177).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antigen affinity purified monoclonal antibody (Catalog # M00177) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70 kDa. The expected band size for SOX9 is at 56 kDa.
Click image to see more details
Figure 2. IHC analysis of SOX9 using anti-SOX9 antibody (M00177).
SOX9 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody (M00177) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SOX9 using anti-SOX9 antibody (M00177).
SOX9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody (M00177) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis of SW480 cells, using SOX9 Antibody .
Click image to see more details
Figure 4. IHC analysis of SOX9 using anti-SOX9 antibody (M00177).
SOX9 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SOX9 Antibody (M00177) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SOX9 (Source: Uniprot.org, NCBI)
Gene Name
SOX9
Full Name
Transcription factor SOX-9
Weight
56.137kDa
Alternative Names
campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SOX9; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9 SOX9 CMD1, CMPD1, SRA1, SRXX2, SRXY10 SRY-box transcription factor 9 transcription factor SOX-9|SRY (sex determining region Y)-box9|SRY (sex-determining region Y)-box 9 protein|SRY-box 9|SRY-related HMG-box, gene 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX9, check out the SOX9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX9 Rabbit Monoclonal Antibody (M00177)
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No publications found for M00177
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-SOX9 Rabbit Monoclonal Antibody
Question
Would anti-SOX9 Rabbit Monoclonal antibody M00177 work for WB with testis?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
According to the expression profile of testis, SOX9 is highly expressed in testis. So, it is likely that anti-SOX9 Rabbit Monoclonal antibody M00177 will work for WB with testis.
Boster Scientific Support
Answered: 2020-05-08
Question
Our lab used your anti-SOX9 Rabbit Monoclonal antibody for IP on testis in a previous experiment. I am using rat, and We are going to use the antibody for IF next. I was wanting to use examining testis as well as eye pns in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
I took a look at the website and datasheets of our anti-SOX9 Rabbit Monoclonal antibody and I see that M00177 has been tested on rat in both IP and IF. Thus M00177 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-07
Question
I see that the anti-SOX9 Rabbit Monoclonal antibody M00177 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-SOX9 Rabbit Monoclonal antibody M00177 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-SOX9 Rabbit Monoclonal antibody (M00177) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
Question
I was wanting to use your anti-SOX9 Rabbit Monoclonal antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
It shows on the product datasheet, M00177 anti-SOX9 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-24
Question
Is this M00177 anti-SOX9 Rabbit Monoclonal antibody reactive to the isotypes of SOX9?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The immunogen of M00177 anti-SOX9 Rabbit Monoclonal antibody is A synthesized peptide derived from human SOX9 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-SOX9 Rabbit Monoclonal antibody M00177. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-23
Question
See below the WB image, lot number and protocol we used for testis using anti-SOX9 Rabbit Monoclonal antibody M00177. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
Can you help my question with product M00177, anti-SOX9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Thomas
Verified customer
Asked: 2019-06-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00177 anti-SOX9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-28
Question
Our lab were content with the WB result of your anti-SOX9 Rabbit Monoclonal antibody. However we have seen positive staining in eye pns nucleus using this antibody. Is that expected? Could you tell me where is SOX9 supposed to be expressed?
M. Zhao
Verified customer
Asked: 2018-11-01
Answer
Based on literature, eye pns does express SOX9. Generally SOX9 expresses in nucleus. Regarding which tissues have SOX9 expression, here are a few articles citing expression in various tissues:
Eye, and PNS, Pubmed ID: 15489334
Testis, Pubmed ID: 7990924
Boster Scientific Support
Answered: 2018-11-01
Question
We have been able to see staining in rat testis. Any tips? Is anti-SOX9 Rabbit Monoclonal antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
Based on literature testis does express SOX9. Based on Uniprot.org, SOX9 is expressed in testis, eye pns, among other tissues. Regarding which tissues have SOX9 expression, here are a few articles citing expression in various tissues:
Eye, and PNS, Pubmed ID: 15489334
Testis, Pubmed ID: 7990924
Boster Scientific Support
Answered: 2018-05-08
Question
Would anti-SOX9 Rabbit Monoclonal antibody M00177 work on pig IP with testis?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
Our lab technicians have not validated anti-SOX9 Rabbit Monoclonal antibody M00177 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SOX9 Rabbit Monoclonal antibody M00177 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig testis in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-23
Question
Does M00177 anti-SOX9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Parker
Verified customer
Asked: 2017-09-27
Answer
You can see on the product datasheet, M00177 anti-SOX9 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-27
Question
We want to test anti-SOX9 Rabbit Monoclonal antibody M00177 on rat testis for research purposes, then I may be interested in using anti-SOX9 Rabbit Monoclonal antibody M00177 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-14
Answer
The products we sell, including anti-SOX9 Rabbit Monoclonal antibody M00177, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-14
Question
Is a blocking peptide available for product anti-SOX9 Rabbit Monoclonal antibody (M00177)?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
We do provide the blocking peptide for product anti-SOX9 Rabbit Monoclonal antibody (M00177). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-31
Question
Is there a BSA free version of anti-SOX9 Rabbit Monoclonal antibody M00177 available?
H. Moore
Verified customer
Asked: 2016-04-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SOX9 Rabbit Monoclonal antibody M00177 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-04-13