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SP100 antibody (A02051-1) performs well in WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of SP100 using anti-SP100 antibody (A02051-1).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP100 antigen affinity purified polyclonal antibody (A02051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SP100 at approximately 80-90 kDa. The expected band size for SP100 is at 55 kDa.
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP100 antigen affinity purified polyclonal antibody (A02051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SP100 at approximately 80-90 kDa. The expected band size for SP100 is at 55 kDa.
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Anti-SP100 Antibody Picoband®
SP100 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | WB |
Tested Applications
SP100 antibody was positively detected by WB in:
rat spleen tissue, rat thymus tissue, rat lung tissue, rat PC-12 whole cell, mouse spleen tissue, mouse thymus tissue, mouse lung tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-SP100 Antibody Picoband®
View all SP100 Antibodies
View all SP100 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SP100.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
80-90 kDa
Calculated Molecular Weight
54.7 kDa
Background of SP100
Predicted to enable several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; chromo shadow domain binding activity; and protein homodimerization activity. Predicted to be involved in several processes, including innate immune response; regulation of DNA-templated transcription; and regulation of signal transduction. Predicted to be located in cytoplasm and nuclear lumen. Predicted to be part of Mre11 complex. Is expressed in eye; olfactory placode; and otic pit. Orthologous to human SP100 (SP100 nuclear antigen).
Cite This Product
Anti-SP100 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02051-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
67%
Low homology
Human
56%
Low homology
Sheep
56%
Low homology
Zebrafish
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SP100 antibody
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A02051-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 30, 2026