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Product Info Summary
| SKU: | PA1865 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor Sp4 Antibody Picoband®
SKU/Catalog Number
PA1865
BA3820-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor Sp4 Antibody catalog # PA1865. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor Sp4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1865)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SP4, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1865 is reactive to SP4 in Human, Mouse, Rat
Observed Molecular Weight
82 kDa
Calculated molecular weight
82.0 kDa
Background of SP4
SP4 (Sp4 transcription factor), also known as Transcription factor Sp4, MGC130008, MGC130009, SPR-1, HF1B, is a protein that in humans is encoded by the SP4 gene. It is a multigene family of SP proteins. Northern blot analysis that SP4 is abundantly expressed as a 7.5-kb message in brain, but barely detectable in other human cell lines. SP4 is an activator protein like SP1. However, in contrast to SP1, SP4 is not able to act synergistically through adjacent binding sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1865 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Sp4 using anti-Sp4 antibody (PA1865).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp4 antigen affinity purified polyclonal antibody (Catalog # PA1865) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sp4 at approximately 82 kDa. The expected band size for Sp4 is at 82 kDa.
Specific Publications For Anti-Transcription factor Sp4 Antibody Picoband® (PA1865)
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Customer Q&As
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3 Customer Q&As for Anti-Transcription factor Sp4 Antibody Picoband®
Question
Does anti-Transcription factor Sp4 antibody PA1865 work on zebrafish WB with uterus?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
Our lab technicians have not tested anti-Transcription factor Sp4 antibody PA1865 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Transcription factor Sp4 antibody PA1865 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-23
Question
Does anti-Transcription factor Sp4 antibody PA1865 work for WB with uterus?
B. Jackson
Verified customer
Asked: 2016-11-01
Answer
According to the expression profile of uterus, SP4 is highly expressed in uterus. So, it is likely that anti-Transcription factor Sp4 antibody PA1865 will work for WB with uterus.
Boster Scientific Support
Answered: 2016-11-01
Question
I was wanting to use your anti-Transcription factor Sp4 antibody for WB for rat uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat uterus identification?
J. Wu
Verified customer
Asked: 2016-01-29
Answer
It shows on the product datasheet, PA1865 anti-Transcription factor Sp4 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-29
