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Product Info Summary
| SKU: | PB9558 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor Sp4 Antibody Picoband®
SKU/Catalog Number
PB9558
PB0594 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor Sp4 Antibody Picoband® catalog # PB9558. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor Sp4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9558)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SP4, different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9558 is reactive to SP4 in Human, Mouse, Rat
Observed Molecular Weight
82 kDa
Calculated molecular weight
82.0 kDa
Background of SP4
SP4 (Sp4 transcription factor), also known as Transcription factor Sp4, MGC130008, MGC130009, SPR-1, HF1B, is a protein that in humans is encoded by the SP4 gene. It is a multigene family of SP proteins. Northern blot analysis that SP4 is abundantly expressed as a 7.5-kb message in brain, but barely detectable in other human cell lines. SP4 is an activator protein like SP1. However, in contrast to SP1, SP4 is not able to act synergistically through adjacent binding sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9558 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, U87 Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
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Western blot analysis of SP4 using anti-SP4 antibody (PB9558).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: U87 Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP4 antigen affinity purified polyclonal antibody (Catalog # PB9558) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP4 at approximately 82 kDa. The expected band size for SP4 is at 82 kDa.
Specific Publications For Anti-Transcription factor Sp4 Antibody Picoband® (PB9558)
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1 Customer Q&As for Anti-Transcription factor Sp4 Antibody Picoband®
Question
We are currently using anti-Transcription factor Sp4 antibody PB9558 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The anti-Transcription factor Sp4 antibody (PB9558) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17
