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Product Info Summary
| SKU: | A00862 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-SPARC Antibody
SKU/Catalog Number
A00862
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SPARC Antibody catalog # A00862. Tested in WB,IHC-P,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPARC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00862)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00862 is reactive to SPARC in Human, Mouse, Rat
Calculated molecular weight
34.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00862 is guaranteed for IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB :1:500-1:2000
IHC-P: 1:50-1:100
ICC/IF: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of A375 cells, using SPARC antibody at 1:3000 dilution.
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Immunofluorescence analysis of C6 cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of L-929 cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U-20S cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens).
Specific Publications For Anti-SPARC Antibody (A00862)
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