Anti-SPARC Antibody Picoband™

Boster Bio Anti-SPARC Antibody Picoband™ catalog # A00862-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00862-2
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-SPARC Antibody Picoband™

See all Sparc products

SKU/Catalog Number

A00862-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SPARC Antibody Picoband™ catalog # A00862-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SPARC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00862-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse SPARC recombinant protein (Position: H123-L300).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00862-2 is reactive to Sparc in Human, Mouse, Rat

Applications

A00862-2 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Sparc (Source: Uniprot.Org, NCBI)

Uniprot ID

P07214

Gene ID

20692

Gene Name

Sparc

Full Name

SPARC

Weight

34.45kDa

Superfamily

SPARC family

Alternative Names

Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC Sparc|BM-40, ON, osteo|secreted acidic cysteine rich glycoprotein|SPARC|basement-membrane protein 40|osteonectin|secreted protein acidic and rich in cysteine

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Sparc, check out the Sparc Infographic

Sparc infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Sparc: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00862-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CD90 is upregulated in gastric cancer tissues and inhibits gastric cancer cell apoptosis by modulating the expression level of SPARC protein

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16 Customer Q&As for Anti-SPARC Antibody Picoband™

Question

My question regarding product A00862-2, anti-SPARC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00862-2 anti-SPARC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-23

Question

Is a blocking peptide available for product anti-SPARC antibody (A00862-2)?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

We do provide the blocking peptide for product anti-SPARC antibody (A00862-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-18

Question

Do you have a BSA free version of anti-SPARC antibody A00862-2 available?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SPARC antibody A00862-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-30

Question

I was wanting to use your anti-SPARC antibody for ELISA for mouse tibia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse tibia identification?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

It shows on the product datasheet, A00862-2 anti-SPARC antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse tibia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-18

Question

Will anti-SPARC antibody A00862-2 work for ELISA with tibia?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

According to the expression profile of tibia, SPARC is highly expressed in tibia. So, it is likely that anti-SPARC antibody A00862-2 will work for ELISA with tibia.

Boster Scientific Support

Answered: 2019-10-04

Question

I see that the anti-SPARC antibody A00862-2 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-23

Question

Here is the WB image, lot number and protocol we used for tibia using anti-SPARC antibody A00862-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-11

Question

Does A00862-2 anti-SPARC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

As indicated on the product datasheet, A00862-2 anti-SPARC antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-26

Question

Is this A00862-2 anti-SPARC antibody reactive to the isotypes of SPARC?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

The immunogen of A00862-2 anti-SPARC antibody is E. coli-derived mouse SPARC recombinant protein (Position: H123-L300). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-23

Question

We are interested in to test anti-SPARC antibody A00862-2 on mouse tibia for research purposes, then I may be interested in using anti-SPARC antibody A00862-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Taylor

Verified customer

Asked: 2019-05-13

Answer

The products we sell, including anti-SPARC antibody A00862-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-13

Question

We have seen staining in mouse tibia. Are there any suggestions? Is anti-SPARC antibody supposed to stain tibia positively?

Verified Customer

Verified customer

Asked: 2018-06-06

Answer

From literature tibia does express SPARC. From Uniprot.org, SPARC is expressed in tibia, placenta, muscle, pns skin, among other tissues. Regarding which tissues have SPARC expression, here are a few articles citing expression in various tissues:
Muscle, PNS, and Skin, Pubmed ID: 15489334
Placenta, Pubmed ID: 3410046

Boster Scientific Support

Answered: 2018-06-06

Question

We bought anti-SPARC antibody for ELISA on placenta a few years ago. I am using mouse, and I plan to use the antibody for WB next. I am looking for examining placenta as well as pns skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-03-09

Answer

I viewed the website and datasheets of our anti-SPARC antibody and I see that A00862-2 has been validated on mouse in both ELISA and WB. Thus A00862-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-03-09

Question

We are currently using anti-SPARC antibody A00862-2 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-17

Answer

The anti-SPARC antibody (A00862-2) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-17

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-SPARC antibody A00862-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-08-21

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-21

Question

We are interested in using your anti-SPARC antibody for r-mmu-3000497; scavenging by class h receptors studies. Has this antibody been tested with western blotting on rat lung tissue? We would like to see some validation images before ordering.

C. Zhao

Verified customer

Asked: 2017-07-25

Answer

We appreciate your inquiry. This A00862-2 anti-SPARC antibody is tested on rat lung tissue, mouse lung tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-07-25

Question

We were well pleased with the WB result of your anti-SPARC antibody. However we have been able to see positive staining in muscle secreted using this antibody. Is that expected? Could you tell me where is SPARC supposed to be expressed?

R. Huang

Verified customer

Asked: 2013-01-03

Answer

From what I have seen in literature, muscle does express SPARC. Generally SPARC expresses in secreted, extracellular space, extracellular. Regarding which tissues have SPARC expression, here are a few articles citing expression in various tissues:
Muscle, PNS, and Skin, Pubmed ID: 15489334
Placenta, Pubmed ID: 3410046

Boster Scientific Support

Answered: 2013-01-03

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A00862-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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