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Product Info Summary
| SKU: | A15127 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPATA4 Antibody
SKU/Catalog Number
A15127
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPATA4 Antibody (Catalog # A15127). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SPATA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPATA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15127)
Host
Rabbit
Contents
SPATA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPATA4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA4. The immunogen is located within amino acids 200 - 250 of SPATA4.
Cross-reactivity
At least two isoforms of SPATA4 are known to exist; this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members
Reactive Species
A15127 is reactive to SPATA4 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
34.8 kDa
Background of SPATA4
The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15127 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 μg/mL.
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Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 μg/mL.
Specific Publications For Anti-SPATA4 Antibody (A15127)
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