Anti-Spermidine synthase/SRM Antibody Picoband™

Product Name

Anti-Spermidine synthase/SRM Antibody Picoband™

View all SRM Antibodies

SKU/Catalog Number

A01594-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Spermidine synthase/SRM Antibody Picoband™ catalog # A01594-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Spermidine synthase/SRM Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01594-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Spermidine synthase/SRM recombinant protein (Position: A15-E292).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01594-2 is reactive to SRM in Human

Applications

A01594-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

33.825kDa

Background of SRM

The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Spermidine synthase/SRM using anti-Spermidine synthase/SRM antibody (A01594-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spermidine synthase/SRM antigen affinity purified polyclonal antibody (Catalog # A01594-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spermidine synthase/SRM at approximately 34 kDa. The expected band size for Spermidine synthase/SRM is at 34 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Spermidine synthase/SRM antibody (A01594-2).
Overlay histogram showing HepG2 cells stained with A01594-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Spermidine synthase/SRM Antibody (A01594-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SRM (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.5.1; EC 2.5.1.16; Putrescine aminopropyltransferase; SPDSY; spermidine synthase; spermidine synthase-1; SPS1PAPT; SRML1 SRM PAPT, SPDSY, SPS1L1, SRM spermidine synthase spermidine synthase|putrescine aminopropyltransferase|spermidine synthase-1

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In this infographic, you will see the following information for SRM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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