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Product Info Summary
| SKU: | A13127 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SPESP1 Antibody
SKU/Catalog Number
A13127
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SPESP1 Antibody catalog # A13127. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-SPESP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13127)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human SPESP1 recombinant protein (Position: E141-R341).
Reactive Species
A13127 is reactive to SPESP1 in Human, Mouse, Rat
Observed Molecular Weight
77 kDa
Calculated molecular weight
38.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13127 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
ELISA, 1:100-1000
Positive Control
WB: human OE19 whole cell lysates,< human MCF-7 whole cell lysates, human RT4 whole cell lysates, human SH-SY5Y whole cell lysates, rat testis tissue lysates, rat C6 whole cell lysates, mouse testis tissue lysates, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of SPESP1 using anti-SPESP1 antibody (A13127).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human OE19 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPESP1 antigen affinity purified polyclonal antibody (A13127) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPESP1 at approximately 77 kDa. The expected band size for SPESP1 is at 39 kDa.
Specific Publications For Anti-SPESP1 Antibody (A13127)
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