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Product Info Summary
| SKU: | A01994-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-SPG3A/ATL1 Antibody Picoband™
SKU/Catalog Number
A01994-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SPG3A/ATL1 Antibody Picoband™ catalog # A01994-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SPG3A/ATL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01994-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SPG3A/ATL1, which shares 93.8% and 100% amino acid (aa) sequence identity with mouse and rat ATL1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01994-1 is reactive to ATL1 in Human
Applications
A01994-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
64 kDa
Calculated molecular weight
63.544kDa
Background of SPG3A
Atlastin, or Atlastin-1, is a protein that in humans is encoded by the ATL1 gene. The protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPG3A/ATL1 antigen affinity purified polyclonal antibody (Catalog # A01994-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPG3A/ATL1 at approximately 64 kDa. The expected band size for SPG3A/ATL1 is at 64 kDa.
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Figure 2. IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1).
SPG3A/ATL1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1).
SPG3A/ATL1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1).
SPG3A/ATL1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 5. IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1).
SPG3A/ATL1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 6. Flow Cytometry analysis of U87 cells using anti-SPG3A/ATL1 antibody (A01994-1).
Overlay histogram showing U87 cells stained with A01994-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPG3A/ATL1 Antibody (A01994-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ATL1 (Source: Uniprot.org, NCBI)
Gene Name
ATL1
Full Name
Atlastin-1
Weight
63.544kDa
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
AD-FSP; atlastin GTPase 1; atlastin1; atlastin-1; Brain-specific GTP-binding protein; EC 3.6.5.-; FSP1; GBP3; GBP-3; GTP-binding protein 3; Guanine nucleotide-binding protein 3; guanylate-binding protein 3; Spastic paraplegia 3 protein A; spastic paraplegia 3A (autosomal dominant); SPG3; SPG3AhGBP3 ATL1 AD-FSP, FSP1, GBP3, HSN1D, SPG3, SPG3A, atlastin1 atlastin GTPase 1 atlastin-1|GBP-3|GTP-binding protein 3|brain-specific GTP-binding protein|guanine nucleotide-binding protein 3|guanylate-binding protein 3|hGBP3|spastic paraplegia 3 protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATL1, check out the ATL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPG3A/ATL1 Antibody Picoband™ (A01994-1)
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