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Product Info Summary
| SKU: | A11403 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPINK2 Antibody
SKU/Catalog Number
A11403
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPINK2 Antibody (Catalog # A11403). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SPINK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPINK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11403)
Host
Rabbit
Contents
SPINK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPINK2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of mouse SPINK2. The immunogen is located within the last 50 amino acids of SPINK2.
Cross-reactivity
SPINK2 antibody is predicted to not cross-react with other SPINK family members.
Reactive Species
A11403 is reactive to SPINK2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
9.7 kDa
Background of SPINK2
Human serine proteinase inhibitor Kazal-type 2 (SPINK2) is required for maintaining normal spermatogenesis and potentially regulates serine protease-mediated apoptosis in male germ cells. It contains a typical kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. SPINK2 plays a role in the pathogenesis of hereditary and chronic pancreatitis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11403 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of SPINK2 in mouse testis tissue with SPINK2 antibody at 2.5 μg/mL.
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Immunofluorescence of SPINK2 in mouse testis tissue with SPINK2 antibody at 20 μg/mL.
Specific Publications For Anti-SPINK2 Antibody (A11403)
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