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Product Info Summary
| SKU: | A03613 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Spred1 Antibody
SKU/Catalog Number
A03613
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Spred1 Antibody (Catalog # A03613). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Spred1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Spred1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03613)
Host
Rabbit
Contents
Spred1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Spred1 antibody was raised against a 20 amino acid synthetic peptide near the center of the human Spred1. The immunogen is located within amino acids 120 - 170 of Spred1.
Reactive Species
A03613 is reactive to SPRED1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.5 kDa
Background of SPRED1
The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03613 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Spred1 in mouse brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 μg/mL.
Specific Publications For Anti-Spred1 Antibody (A03613)
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