Anti-Spt6/SUPT6H Antibody Picoband™

Product Name

Anti-Spt6/SUPT6H Antibody Picoband™

View all Spt6 Antibodies

SKU/Catalog Number

A06484-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Spt6/SUPT6H Antibody Picoband™ catalog # A06484-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Spt6/SUPT6H Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06484-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Spt6/SUPT6H recombinant protein (Position: A564-Q1639).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06484-1 is reactive to SUPT6H in Human, Monkey, Mouse, Rat

Applications

A06484-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

260 kDa

Calculated molecular weight

199.073kDa

Background of Spt6

Transcription elongation factor SPT6 is a protein that in humans is encoded by the SUPT6H gene. SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and directly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Spt6/SUPT6H using anti-Spt6/SUPT6H antibody (A06484-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spt6/SUPT6H antigen affinity purified polyclonal antibody (Catalog # A06484-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spt6/SUPT6H at approximately 260 kDa. The expected band size for Spt6/SUPT6H is at 199 kDa.

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Figure 2. Flow Cytometry analysis of U251 cells using anti-Spt6/SUPT6H antibody (A06484-1).
Overlay histogram showing U251 cells stained with A06484-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Spt6/SUPT6H Antibody (A06484-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SUPT6H (Source: Uniprot.org, NCBI)

Alternative Names

emb-5; hSPT6; KIAA0162MGC87943; SPT6HSPT6; suppressor of Ty (S.cerevisiae) 6 homolog; suppressor of Ty 6 homolog (S. cerevisiae); Tat-cotransactivator 2 protein; Tat-CT2 protein; transcription elongation factor SPT6 SUPT6H SPT6, SPT6H, emb-5 SPT6 homolog, histone chaperone and transcription elongation factor transcription elongation factor SPT6|histone chaperone suppressor of Ty6|suppressor of Ty 6 homolog|tat-CT2 protein|tat-cotransactivator 2 protein

For more info on SUPT6H, check out the SUPT6H Infographic

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In this infographic, you will see the following information for SUPT6H: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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