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Product Info Summary
| SKU: | A06484 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-SUPT6H Antibody
SKU/Catalog Number
A06484
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SUPT6H Antibody catalog # A06484. Tested in WB,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SUPT6H Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06484)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human SUPT6H(NP_003161.2).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06484 is reactive to SUPT6H in Human, Mouse, Rat
Calculated molecular weight
199.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06484 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using SUPT6H antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
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Immunofluorescence analysis of U-2 OS cells using SUPT6H antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 600ug extracts of Mouse heart using 3ug SUPT6H antibody . Western blot was performed from the immunoprecipitate using SUPT6H antibody at a dilution of 1:500.
Specific Publications For Anti-SUPT6H Antibody (A06484)
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