Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody

Product Name

Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody

View all SPTLC1 Antibodies

SKU/Catalog Number

PA1777

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody catalog # PA1777. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1777)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SPTLC1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1777 is reactive to SPTLC1 in Human

Applications

PA1777 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

52.744kDa

Background of SPTLC1

SPTLC1 (Serine palmitoyltransferase, long chain base subunit 1), also known as SPT1, LCB1, is a protein which in humans is encoded by the SPTLC1 gene. Dawkins et al. (2001)noted that the SPTLC1 gene maps to chromosome 9q22.1-q22.3. Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-SPTLC1 antibody, PA1777, Western blotting
All lanes: Anti SPTLC1 (PA1777) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: S549 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD

Click image to see more details

Anti-SPTLC1 antibody, PA1777, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Gene/Protein Information For SPTLC1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.3.1.50; hereditary sensory neuropathy, type 1; HSAN1; LBC1; LCB 1; LCB1HSAN; Long chain base biosynthesis protein 1; MGC14645; serine C-palmitoyltransferase; serine palmitoyltransferase 1; serine palmitoyltransferase, long chain base subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1HSN1; SPTI SPTLC1 HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI serine palmitoyltransferase long chain base subunit 1 serine palmitoyltransferase 1|long chain base biosynthesis protein 1|serine C-palmitoyltransferase|serine-palmitoyl-CoA transferase 1

For more info on SPTLC1, check out the SPTLC1 Infographic

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In this infographic, you will see the following information for SPTLC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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