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Product Info Summary
| SKU: | A10463-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-SR-1F HTR1F Antibody
SKU/Catalog Number
A10463-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SR-1F HTR1F Antibody catalog # A10463-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SR-1F HTR1F Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10463-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human 5-HT-1F. AA range:101-150
Reactive Species
A10463-1 is reactive to HTR1F in Human, Monkey, Mouse, Rat
Calculated molecular weight
41.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10463-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of various cells using SR-1F Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from COS7 cells, using 5-HT-1F Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-SR-1F HTR1F Antibody (A10463-1)
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Customer Q&As
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7 Customer Q&As for Anti-SR-1F HTR1F Antibody
Question
Is a blocking peptide available for product anti-SR-1F antibody (A10463-1)?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We do provide the blocking peptide for product anti-SR-1F antibody (A10463-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-SR-1F antibody A10463-1 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-SR-1F antibody (A10463-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
Question
I have a question about product A10463-1, anti-SR-1F antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Lewis
Verified customer
Asked: 2019-05-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10463-1 anti-SR-1F antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SR-1F antibody A10463-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-01
Question
Does A10463-1 anti-SR-1F antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Patel
Verified customer
Asked: 2015-05-18
Answer
It shows on the product datasheet, A10463-1 anti-SR-1F antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-18
Question
I was wanting to use your anti-SR-1F antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
B. Miller
Verified customer
Asked: 2014-12-25
Answer
It shows on the product datasheet, A10463-1 anti-SR-1F antibody has been validated for WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-25
Question
Is this A10463-1 anti-SR-1F antibody reactive to the isotypes of HTR1F?
D. Bhatt
Verified customer
Asked: 2013-05-09
Answer
The immunogen of A10463-1 anti-SR-1F antibody is Synthesized peptide derived from the Internal region of human SR-1F. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-05-09
