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Product Info Summary
| SKU: | A08082 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-SRD5A3 Antibody (C-term)
SKU/Catalog Number
A08082
Size
80 µl
Description
Boster Bio Anti-SRD5A3 Antibody (C-term) (Catalog # A08082). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SRD5A3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08082)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SRD5A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human SRD5A3.
Reactive Species
A08082 is reactive to SRD5A3 in Human
Calculated molecular weight
36.5 kDa
Background of SRD5A3
SRD5A3 converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08082 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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SRD5A3 Antibody (C-term) western blot analysis in 293, LNCaP, PC-3, NCI-H292 cell line lysates (35ug/lane). This demonstrates the SRD5A3 antibody detected the SRD5A3 protein (arrow).
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SRD5A3 Antibody (C-term) (Cat. #A08082) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SRD5A3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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SRD5A3 Antibody (C-term) (Cat. #A08082) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-SRD5A3 Antibody (C-term) (A08082)
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