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- Table of Contents
STC1 antibody (A03402) performs well in ELISA, IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of Stanniocalcin 1/STC/STC1 using anti-Stanniocalcin 1/STC/STC1 antibody (A03402).
Stanniocalcin 1/STC/STC1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Stanniocalcin 1/STC/STC1 Antibody (A03402) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Stanniocalcin 1/STC/STC1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Stanniocalcin 1/STC/STC1 Antibody (A03402) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Stanniocalcin 1/STC/STC1 Antibody
STC1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC |
Tested Applications
STC1 antibody was positively detected by IHC in:
human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
ELISA
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Stanniocalcin 1/STC/STC1 Antibody
View all STC1 Antibodies
View all STC1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Stanniocalcin 1/STC/STC1 recombinant protein (Position: V34-A247).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
27.6 kDa
Background of STC1
STC1(Stanniocalcin 1), also called STC, which is encoded by the STC1 gene. By fluorescence in situ hybridization, Chang et al.(1998) mapped the STC1 gene to chromosome 8p21-p11.2. Wagner et al.(1995) found evidence of STC immunoreactivity in human kidney and serum, suggesting the existence of the hormone in mammals. Zhang et al.(1998) reported a constitutive expression of STC in terminally differentiated neurons. Bell et al.(2001) determined that STC1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices.
Cite This Product
Anti-Stanniocalcin 1/STC/STC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03402)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
98%
High Homology
Mouse
98%
High Homology
Rat
97%
High Homology
Sheep
96%
High Homology
Cow
95%
High Homology
Rabbit
81%
Moderate Homology
Zebrafish
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for STC1 antibody
Publications
Application:
Species:
Stanniocalcin-1 augments stem-like traits of glioblastoma cells through binding and activating NOTCH1
Published: 2017-11-28
Journal: CANCER LETTERS
Application: IF
Impact Factor: 6.38
Species: Human
PMID: 29196129
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Product Promise
We promise Anti-Stanniocalcin 1/STC/STC1 Antibody performs excellently in relevant applications for ELISA, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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A03402
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026