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Product Info Summary
| SKU: | A03402 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Stanniocalcin 1/STC/STC1 Antibody
SKU/Catalog Number
A03402
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Stanniocalcin 1/STC/STC1 Antibody catalog # A03402. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Stanniocalcin 1/STC/STC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03402)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Stanniocalcin 1/STC/STC1 recombinant protein (Position: V34-A247).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03402 is reactive to STC1 in Human
Calculated molecular weight
27.6 kDa
Background of STC1
STC1(Stanniocalcin 1), also called STC, which is encoded by the STC1 gene. By fluorescence in situ hybridization, Chang et al.(1998) mapped the STC1 gene to chromosome 8p21-p11.2. Wagner et al.(1995) found evidence of STC immunoreactivity in human kidney and serum, suggesting the existence of the hormone in mammals. Zhang et al.(1998) reported a constitutive expression of STC in terminally differentiated neurons. Bell et al.(2001) determined that STC1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03402 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
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IHC analysis of Stanniocalcin 1/STC/STC1 using anti-Stanniocalcin 1/STC/STC1 antibody (A03402).
Stanniocalcin 1/STC/STC1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Stanniocalcin 1/STC/STC1 Antibody (A03402) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Stanniocalcin 1/STC/STC1 Antibody (A03402)
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