Anti-STAT3 Rabbit Monoclonal Antibody

Product Name

Anti-STAT3 Rabbit Monoclonal Antibody

View all STAT3 Antibodies

SKU/Catalog Number

M00007

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-STAT3 Rabbit Monoclonal Antibody catalog # M00007. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STAT3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00007)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AIH-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human STAT3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00007 is reactive to STAT3 in Human, Mouse, Rat

Applications

M00007 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

88.068kDa

Background of STAT3

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:200-1:500

Validation Images & Assay Conditions

Click image to see more details

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Click image to see more details

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Click image to see more details

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Click image to see more details

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Click image to see more details

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Click image to see more details

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Click image to see more details

Western blot analysis of STAT3 expression in (1) A431 cell lysate; (2) Raji cell lysate.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using STAT3 Antibody.

Click image to see more details

Immunofluorescent analysis of HeLa cells, using STAT3 Antibody.

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Gene/Protein Information For STAT3 (Source: Uniprot.org, NCBI)

Alternative Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3 STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor

For more info on STAT3, check out the STAT3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for STAT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the STAT3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis