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Product Info Summary
| SKU: | M04259 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Staufen STAU1 Monoclonal Antibody
SKU/Catalog Number
M04259
BM5206 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Staufen STAU1 Monoclonal Antibody catalog # M04259. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Staufen STAU1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04259)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACAC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Staufen Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
Reactive Species
M04259 is reactive to STAU1 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
63.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04259 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human K562 whole cell, human 293T whole cell, human HepG2 whole cell, human Caco-2 whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of STAU1 using anti-STAU1 antibody (M04259).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAU1 antigen affinity purified monoclonal antibody (M04259) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAU1 at approximately 55 kDa. The expected band size for STAU1 is at 63 kDa.
Specific Publications For Anti-Staufen STAU1 Monoclonal Antibody (M04259)
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Customer Q&As
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7 Customer Q&As for Anti-Staufen STAU1 Monoclonal Antibody
Question
I was wanting to use your anti-Staufen Monoclonal antibody for WB for rat cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma erythroleukemia identification?
J. Parker
Verified customer
Asked: 2019-10-18
Answer
As indicated on the product datasheet, M04259 anti-Staufen Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-18
Question
Do you have a BSA free version of anti-Staufen Monoclonal antibody M04259 available?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Staufen Monoclonal antibody M04259 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-17
Question
Our lab want to know about to test anti-Staufen Monoclonal antibody M04259 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Staufen Monoclonal antibody M04259 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
The products we sell, including anti-Staufen Monoclonal antibody M04259, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-07
Question
I see that the anti-Staufen Monoclonal antibody M04259 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-02-14
Question
Is this M04259 anti-Staufen Monoclonal antibody reactive to the isotypes of STAU1?
J. Mangal
Verified customer
Asked: 2018-02-08
Answer
The immunogen of M04259 anti-Staufen Monoclonal antibody is A synthesized peptide derived from human Staufen Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-08
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Staufen Monoclonal antibody M04259. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-05
Question
I have a question about product M04259, anti-Staufen Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Li
Verified customer
Asked: 2015-06-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04259 anti-Staufen Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-12
