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Product Info Summary
| SKU: | A09073 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Stella DPPA3 Antibody
SKU/Catalog Number
A09073
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Stella DPPA3 Antibody (Catalog # A09073). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Stella antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Stella DPPA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09073)
Host
Rabbit
Contents
Stella Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Stella antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Stella. The immunogen is located within the last 50 amino acids of Stella.
Reactive Species
A09073 is reactive to DPPA3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
17.9 kDa
Background of DPPA3
Stella was initially identified in primordial germ cells and pre-implantation embryos whose expression as a maternal factor is important in early embryonic development but is not required for germ cell specification in mice. In humans, Stella is thought to be a marker for pluripotency in embryonic stem (ES) cells as its expression is observed in primordial germ cells of both sexes and germ cell tumors but not in normal somatic tissues. However, in ES cell colonies, heterogeneous expression of Stella was seen in high throughput in situ hybridization assays, indicating that higher levels of complexity exist in otherwise thought to be undifferentiated ES cells. At least two distinct isoforms of Stella are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09073 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Stella antibody can be used for detection of Stella by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Stella in 293 cell lysate with Stella antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-Stella DPPA3 Antibody (A09073)
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