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Product Info Summary
| SKU: | A30516 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-STRAD Antibody
SKU/Catalog Number
A30516
Size
100ul
Form
Liquid
Description
Boster Bio Anti-STRAD Antibody (Catalog# A30516). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STRAD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30516)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human STRAD. Immunogen sequence location: 11-60
Reactive Species
A30516 is reactive to STRADA in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
48.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30516 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200
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Western Blot analysis of various cells using Strad Polyclonal Antibody
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Western blot analysis of lysates from HepG2 cells, using STRAD Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-STRAD Antibody (A30516)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-STRAD Antibody
Question
We are currently using anti-STRAD antibody A30516 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The anti-STRAD antibody (A30516) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-10
Question
Will anti-STRAD antibody A30516 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
According to the expression profile of testis, STRADA is highly expressed in testis. So, it is likely that anti-STRAD antibody A30516 will work for WB with testis.
Boster Scientific Support
Answered: 2019-06-05
Question
Is a blocking peptide available for product anti-STRAD antibody (A30516)?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
We do provide the blocking peptide for product anti-STRAD antibody (A30516). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-11-21
Question
Would anti-STRAD antibody A30516 work on zebrafish WB with eco:0000312?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
Our lab technicians have not tested anti-STRAD antibody A30516 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-STRAD antibody A30516 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish eco:0000312 in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-29
Question
We are interested in to test anti-STRAD antibody A30516 on mouse testis for research purposes, then I may be interested in using anti-STRAD antibody A30516 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Rodriguez
Verified customer
Asked: 2018-01-12
Answer
The products we sell, including anti-STRAD antibody A30516, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-12
