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Product Info Summary
| SKU: | A01112-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-STRADA Antibody (C-term)
SKU/Catalog Number
A01112-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-STRADA Antibody (C-term) (Catalog # A01112-2). Tested in WB, IHC-P, IF, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-STRADA Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01112-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This STRADA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 303-346 amino acids from the C-terminal region of human STRADA.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01112-2 is reactive to STRADA in Human, Mouse
Calculated molecular weight
48.4 kDa
Background of STRADA
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01112-2 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IF: 1:25
IHC-P: 1:100
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-STRADA Antibody (C-term) at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: Molt-4 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Fluorescent image of A549 cells stained with STRADA Antibody (C-term). A01112-2 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Click image to see more details
All lanes : Anti-STRADA Antibody (C-term) at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Molt-4 cell lysate
Lane 3: Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of A01112-2 on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Flow cytometric analysis of SH-SY5Y cells using STRADA Antibody (C-term)(green, Cat#A01112-2) compared to an isotype control of rabbit IgG(blue). A01112-2 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-STRADA Antibody (C-term) (A01112-2)
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