Anti-Stromal interaction molecule 2 STIM2 Antibody

Product Name

Anti-Stromal interaction molecule 2 STIM2 Antibody

View all STIM2 Antibodies

SKU/Catalog Number

A02345-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Stromal interaction molecule 2 STIM2 Antibody (Catalog # A02345-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

STIM2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Stromal interaction molecule 2 STIM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02345-2)

Host

Rabbit

Contents

STIM2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

STIM2 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STIM2. The immunogen is located within the last 50 amino acids of STIM2.

Reactive Species

A02345-2 is reactive to STIM2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

84.0 kDa

Background of STIM2

In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02345-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information STIM2

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