Anti-SUGT1 Antibody Picoband™

Product Name

Anti-SUGT1 Antibody Picoband™

View all SUGT1 Antibodies

SKU/Catalog Number

A05411-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SUGT1 Antibody Picoband™ catalog # A05411-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SUGT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05411-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SUGT1 recombinant protein (Position: D24-E296).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05411-1 is reactive to SUGT1 in Human

Applications

A05411-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

41.024kDa

Background of SUGT1

Suppressor of G2 allele of SKP1 homolog is a protein that in humans is encoded by the SUGT1 gene. This gene encodes a highly conserved nuclear protein involved in kinetochore function and required for the G1/S and G2/M transitions. This protein interacts with heat shock protein 90. Alternative splicing results in multiple transcript variants. Pseudogenes for this gene have been defined on several different chromosomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SUGT1 using anti-SUGT1 antibody (A05411-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUGT1 antigen affinity purified polyclonal antibody (Catalog # A05411-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUGT1 at approximately 36 kDa. The expected band size for SUGT1 is at 41 kDa.

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Figure 2. IF analysis of SUGT1 using anti-SUGT1 antibody (A05411-1) and anti-Tubulin Alpha antibody (M03989-3).
SUGT1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUGT1 Antibody (A05411-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U251 cells using anti-SUGT1 antibody (A05411-1).
Overlay histogram showing U251 cells stained with A05411-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUGT1 Antibody (A05411-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SUGT1 (Source: Uniprot.org, NCBI)

Alternative Names

Protein 40-6-3; putative 40-6-3 protein; SGT1; SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae); SGT1B protein; suppressor of G2 allele of SKP1 homolog; suppressor of G2 allele of SKP1, S. cerevisiae, homolog of SUGT1 SGT1 SGT1 homolog, MIS12 kinetochore complex assembly cochaperone protein SGT1 homolog|SGT1, suppressor of G2 allele of SKP1|putative 40-6-3 protein|suppressor of G2 allele of SKP1, S. cerevisiae, homolog of

For more info on SUGT1, check out the SUGT1 Infographic

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In this infographic, you will see the following information for SUGT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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