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Product Info Summary
| SKU: | M05748 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-SULT2B1
SKU/Catalog Number
M05748
Size
50 µl
Description
Boster Bio Anti-SULT2B1 (Catalog # M05748). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SULT2B1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05748)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2142CT1553.26.63
Isotype
IgG1,κ
Immunogen
This antibody is generated from a mouse immunized with a reconbinant protein from human.
Reactive Species
M05748 is reactive to SULT2B1 in Human
Calculated molecular weight
41.3 kDa
Background of SULT2B1
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation (PubMed:28575648).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05748 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
Validation Images & Assay Conditions
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All lanes : Anti-SULT2B1 at 1:2000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: MDA-MB-468 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-SULT2B1 at 1:8000 dilution + MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-SULT2B1 (M05748)
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