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SURF2 antibody (A14641) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of U937 cells using anti-SURF2 antibody (A14641).
Overlay histogram showing U937 cells stained with A14641 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF2 Antibody (A14641, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U937 cells stained with A14641 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF2 Antibody (A14641, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SURF2 Antibody Picoband®
SURF2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
SURF2 antibody was positively detected by WB in:
human PC-3 whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SURF2 antibody was positively detected by ICC/IF in:
MCF-7 cell
SURF2 antibody was positively detected by FC in:
U937 cell, 293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-SURF2 Antibody Picoband®
View all SURF2 Antibodies
View all SURF2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SURF2 recombinant protein (Position: D7-K230).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
29.6 kDa
Background of SURF2
SURF2 is a protein which in humans is encoded by the SURF2 gene. Surfeit 2, also known as SURF2, belongs to the SURF2 family and interacts with beta-1, 4-Gal-T3, uPAR and WDR20. SURF2 is located in the surfeit gene cluster, which is a group of very tightly linked genes that do not share sequence similarity. The SURF2 gene maps to human chromosome 9q34.2 and shares a bidirectional promoter with SURF1, which is located on the opposite strand. The intergenic region between the SURF1 and SURF2 genes is expected to have bidirectional promoter activity, as is found in mouse. Recombinant human SURF2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Cite This Product
Anti-SURF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14641)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Cow
79%
Moderate Homology
Sheep
79%
Moderate Homology
Dog
76%
Moderate Homology
Mouse
70%
Moderate Homology
Rat
69%
Low homology
Zebrafish
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SURF2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SURF2 antibody
Flow Cytometry (FC) protocol for SURF2 antibody
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Product Promise
We promise Anti-SURF2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A14641
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Nov 1, 2026