Anti-SURF2 Antibody Picoband™

Product Name

Anti-SURF2 Antibody Picoband™

View all SURF2 Antibodies

SKU/Catalog Number

A14641

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SURF2 Antibody Picoband™ catalog # A14641. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SURF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14641)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SURF2 recombinant protein (Position: D7-K230).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A14641 is reactive to SURF2 in Human

Applications

A14641 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

29.648kDa

Background of SURF2

SURF2 is a protein which in humans is encoded by the SURF2 gene. Surfeit 2, also known as SURF2, belongs to the SURF2 family and interacts with beta-1, 4-Gal-T3, uPAR and WDR20. SURF2 is located in the surfeit gene cluster, which is a group of very tightly linked genes that do not share sequence similarity. The SURF2 gene maps to human chromosome 9q34.2 and shares a bidirectional promoter with SURF1, which is located on the opposite strand. The intergenic region between the SURF1 and SURF2 genes is expected to have bidirectional promoter activity, as is found in mouse. Recombinant human SURF2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SURF2 using anti-SURF2 antibody (A14641).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF2 antigen affinity purified polyclonal antibody (Catalog # A14641) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF2 at approximately 38 kDa. The expected band size for SURF2 is at 30 kDa.

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Figure 2. IF analysis of SURF2 using anti-SURF2 antibody (A14641) and anti-Tubulin Alpha antibody (M03989-3).
SURF2 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SURF2 Antibody (A14641) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U937 cells using anti-SURF2 antibody (A14641).
Overlay histogram showing U937 cells stained with A14641 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF2 Antibody (A14641, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 4. Flow Cytometry analysis of 293T cells using anti-SURF2 antibody (A14641).
Overlay histogram showing 293T cells stained with A14641 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF2 Antibody (A14641, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SURF2 (Source: Uniprot.org, NCBI)

Alternative Names

SURF-2; surfeit 2; surfeit locus protein 2 SURF2 SURF-2 surfeit 2 surfeit locus protein 2

For more info on SURF2, check out the SURF2 Infographic

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In this infographic, you will see the following information for SURF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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