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SURF6 antibody (A11571-1) performs well in ELISA, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of SURF6 using anti-SURF6 antibody (A11571-1) and anti-Beta Tubulin antibody (M01857-3).
SURF6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SURF6 Antibody (A11571-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SURF6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SURF6 Antibody (A11571-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-SURF6 Antibody Picoband®
SURF6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, ICC, WB |
Tested Applications
SURF6 antibody was positively detected by WB in:
human Jurkat whole cell, human Hela whole cell, human MCF-7 whole cell, human Hacat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SURF6 antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-SURF6 Antibody Picoband®
View all SURF6 Antibodies
View all SURF6 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human SURF6 recombinant protein (Position: M1-R216). Human SURF6 shares 62.5% amino acid (aa) sequence identity with mouse SURF6.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
41.5 kDa
Background of SURF6
Surfeit locus protein 6 is a protein that in humans is encoded by the SURF6 gene. This gene encodes a conserved protein that is localized to the nucleolus. The encoded protein may function as a nucleolar-matrix protein with nucleic acid-binding properties. There is a pseudogene for this gene on chromosome Y. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-SURF6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11571-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
84%
Moderate Homology
Cow
69%
Low homology
Sheep
67%
Low homology
Dog
65%
Low homology
Mouse
63%
Low homology
Rat
53%
Low homology
Zebrafish
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SURF6 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SURF6 antibody
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Product Promise
We promise Anti-SURF6 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A11571-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026