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RBM12 antibody (A13929) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RT4 cells using anti-SWAN/RBM12 antibody (A13929).
Overlay histogram showing RT4 cells stained with A13929 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SWAN/RBM12 Antibody (A13929, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RT4 cells stained with A13929 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SWAN/RBM12 Antibody (A13929, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SWAN/RBM12 Antibody Picoband®
RBM12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
RBM12 antibody was positively detected by WB in:
human Hela whole cell, human MOLT-4 whole cell, human Daudi whole cell, rat brain tissue, mouse thymus tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RBM12 antibody was positively detected by FC in:
RT4 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-SWAN/RBM12 Antibody Picoband®
View all RBM12 Antibodies
View all RBM12 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SWAN/RBM12 recombinant protein (Position: Q287-E909).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
97 kDa
Calculated Molecular Weight
97.4 kDa
Background of RBM12
RNA-binding protein 12 is a protein that in humans is encoded by the RBM12 gene. This gene encodes a protein that contains several RNA-binding motifs, potential transmembrane domains, and proline-rich regions. This gene and the gene for copine I overlap at map location 20q11.21. Alternative splicing in the 5' UTR results in four transcript variants. All variants encode the same protein.
Cite This Product
Anti-SWAN/RBM12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13929)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Mouse
91%
High Homology
Zebrafish
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for RBM12 antibody
Flow Cytometry (FC) protocol for RBM12 antibody
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Product Promise
We promise Anti-SWAN/RBM12 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A13929
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026