Anti-synaptophysin 2 Synpr Antibody

Product Name

Anti-synaptophysin 2 Synpr Antibody

View all Synaptoporin Antibodies

SKU/Catalog Number

A11201

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-synaptophysin 2 Synpr Antibody (Catalog # A11201). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-synaptophysin 2 Synpr Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11201)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Clone Number

3A8

Isotype

IgG

Immunogen

Peptide sequence around aa.257~261(G-P-T-S-F)derived from Rat synaptophysin 2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11201 is reactive to Synpr in Human, Mouse, Rat

Applications

A11201 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29.107kDa

Background of Synaptoporin

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).

Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
Valtorta, F. et al. (2004) Bioessays 26, 445-53.
Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 37kd
Western blotting: 1:500~1:1000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Synpr using anti-Synpr antibody (A11201).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synpr antigen affinity purified polyclonal antibody (Catalog # A11201) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Synpr.

Gene/Protein Information For Synpr (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686G0883; MGC26651; SPO; synaptoporin Synpr|synaptoporin|synaptoporin|synaptorin

For more info on Synpr, check out the Synpr Infographic

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In this infographic, you will see the following information for Synpr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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