Anti-Synaptophysin-like protein 2 SYPL2 Antibody

Product Name

Anti-Synaptophysin-like protein 2 SYPL2 Antibody

View all SYPL2 Antibodies

SKU/Catalog Number

A14531

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Synaptophysin-like protein 2 SYPL2 Antibody (Catalog # A14531). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SYPL2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Synaptophysin-like protein 2 SYPL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14531)

Host

Rabbit

Contents

SYPL2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SYPL2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SYPL. The immunogen is located within the last 50 amino acids of SYPL2.

Cross-reactivity

At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.

Reactive Species

A14531 is reactive to SYPL2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

30.2 kDa

Background of SYPL2

SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14531 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SYPL2 antibody can be used for detection of SYPL2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SYPL2

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