This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
SYT10 antibody (A14108-2) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IF analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2).
Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Bundle Save
Anti-Synaptotagmin-10/SYT10 Antibody Picoband®
SYT10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Tested Applications
SYT10 antibody was positively detected by WB in:
human MCF-7 whole cell, human RT4 whole cell, human K562 whole cell, human U2OS whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SYT10 antibody was positively detected by IHC in:
rat brain tissue, mouse brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SYT10 antibody was positively detected by IF in:
rat brain tissue, mouse brain tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Mouse, Rat
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
ELISA
Human
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Synaptotagmin-10/SYT10 Antibody Picoband®
View all SYT10 Antibodies
View all SYT10 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Synaptotagmin-10/SYT10 recombinant protein (Position: M1-D363). Human Synaptotagmin-10/SYT10 shares 90.6% and 91.2% amino acid (aa) sequence identity with mouse and rat Synaptotagmin-10/SYT10, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
59 kDa
Calculated Molecular Weight
59.1 kDa
Background of SYT10
Predicted to enable several functions, including calcium ion binding activity; calcium ion sensor activity; and calcium-dependent phospholipid binding activity. Predicted to be involved in positive regulation of calcium ion-dependent exocytosis; presynaptic dense core vesicle exocytosis; and sensory perception of smell. Predicted to be located in transport vesicle membrane. Predicted to be active in exocytic vesicle and plasma membrane.
Cite This Product
Anti-Synaptotagmin-10/SYT10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14108-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
96%
High Homology
Sheep
92%
High Homology
Cow
92%
High Homology
Rat
91%
High Homology
Mouse
91%
High Homology
Rabbit
82%
Moderate Homology
Zebrafish
70%
Moderate Homology
Drosophila
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SYT10 antibody
Immunohistochemistry (IHC) protocol for SYT10 antibody
Immunofluorescence (IF) protocol for SYT10 antibody
Review
Have you used Anti-Synaptotagmin-10/SYT10 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Synaptotagmin-10/SYT10 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A14108-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026