Click image to see more details
Product Info Summary
| SKU: | A06159-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Syncoilin/Sync Antibody Picoband®
SKU/Catalog Number
A06159-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Syncoilin/Sync Antibody Picoband® catalog # A03863-3. Tested in WB, FCM, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Syncoilin/Sync Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06159-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived mouse Syncoilin/Sync recombinant protein (Position: D11-Q465). Mouse Sync shares 76.6% amino acid (aa) sequence identity with human Sync.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06159-2 is reactive to Sync in Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
53.6 kDa
Background of Sync
Syncoilin is a muscle-specific atypical type III intermediate filament protein encoded in the human by the gene SYNC. This gene encodes a member of the intermediate filament family which contains an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. The protein is highly expressed in skeletal and cardiac muscle. The protein links the dystrophin associated protein complex (DAPC) to desmin filaments in muscle and may have a structural role in striated muscle. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06159-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat skeletal muscle tissue, rat heart tissue, rat H9C2(2-1) whole cell, rat L6 whole cell, mouse heart tissue, mouse C2C12 whole cell
FCM: C2C12 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Syncoilin/Sync using anti-Syncoilin/Sync antibody (A06159-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat H9C2(2-1) whole cell lysates,
Lane 4: rat L6 whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syncoilin/Sync antigen affinity purified polyclonal antibody (Catalog # A06159-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syncoilin/Sync at approximately 72 kDa. The expected band size for Syncoilin/Sync is at 54 kDa.
Click image to see more details
Flow Cytometry analysis of C2C12 cells using anti-Syncoilin/Sync antibody (A06159-2).
Overlay histogram showing C2C12 cells stained with A06159-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syncoilin/Sync Antibody (A06159-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-Syncoilin/Sync Antibody Picoband® (A06159-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Syncoilin/Sync Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Syncoilin/Sync Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
