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- Table of Contents
SYNGAP1 antibody (M04862) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of SYNGAP using anti-SYNGAP antibody (M04862).
SYNGAP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti-SYNGAP Antibody (M04862) at a dilution of 1:50 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SYNGAP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti-SYNGAP Antibody (M04862) at a dilution of 1:50 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-SynGAP Rabbit Monoclonal Antibody
SYNGAP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
SYNGAP1 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SYNGAP1 antibody was positively detected by ICC/IF in:
human brain
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Recommend
Tested
Tested
Recommend
Product Information
Product Name
Anti-SynGAP Rabbit Monoclonal Antibody
View all SYNGAP1 Antibodies
View all SYNGAP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEOC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SynGAP
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
148 kDa
Calculated Molecular Weight
148.3 kDa
Cite This Product
Anti-SynGAP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04862)
Protocols
Western Blot protocol for SYNGAP1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SYNGAP1 antibody
Review
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Product Promise
We promise Anti-SynGAP Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M04862
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026