Product Info Summary
SKU: | A02847-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-TAB1 Antibody Picoband™
SKU/Catalog Number
A02847-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAB1 Antibody Picoband™ catalog # A02847-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TAB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02847-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TAB1 recombinant protein (Position: R5-A329).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02847-1 is reactive to TAB1 in Human
Applications
A02847-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
55-65 kDa
Calculated molecular weight
54.644kDa
Background of TAB1
Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, also called TAB1, is an enzyme that in humans is encoded by the MAP3K7IP1 gene. It is mapped to 22q13.1. The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways. This protein can interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. TAB1 also can activate the kinase activity of TAK1 by direct interaction, and it may be an important signaling intermediate between TGFB receptors and TAK1. What's more, TAB1 plays an important role in skin homeostasis, wound repair, and oncogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TAB1 using anti-TAB1 antibody (A02847-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB1 antigen affinity purified polyclonal antibody (Catalog # A02847-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB1 at approximately 55-65 kDa. The expected band size for TAB1 is at 55 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TAB1 (Source: Uniprot.org, NCBI)
Gene Name
TAB1
Full Name
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1
Weight
54.644kDa
Alternative Names
MAP3K7IP1; MAP3K7IP13'-Tab1; MGC57664; mitogen-activated protein kinase kinase kinase 7 interacting protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TAB1; TAK1-binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TGF-beta-activated kinase 1-binding protein 1; transforming growth factor beta-activated kinase-binding protein 1 TAB1 3-Tab1, MAP3K7IP1 TGF-beta activated kinase 1 (MAP3K7) binding protein 1 TGF-beta-activated kinase 1 and MAP3K7-binding protein 1|TAK1-binding protein 1|mitogen-activated protein kinase kinase kinase 7-interacting protein 1|transforming growth factor beta-activated kinase-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAB1, check out the TAB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAB1 Antibody Picoband™ (A02847-1)
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