Anti-TAB2 Antibody Picoband™

Product Name

Anti-TAB2 Antibody Picoband™

View all TAB2 Antibodies

SKU/Catalog Number

A02499-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TAB2 Antibody Picoband™ catalog # A02499-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TAB2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02499-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TAB2 recombinant protein (Position: Q169-K522).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02499-1 is reactive to TAB2 in Human

Applications

A02499-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

76 kDa

Calculated molecular weight

76.494kDa

Background of TAB2

Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, also known as TAB2, is an enzyme that in humans is encoded by the MAP3K7IP2 gene. The protein encoded by this gene is an activator of MAP3K7/TAK1, which is required for the IL-1 induced activation of nuclear factor kappaB and MAPK8/JNK. This protein forms a kinase complex with TRAF6, MAP3K7 and TAB1, thus serves as an adaptor linking MAP3K7 and TRAF6. TAB2 functionally links TAK1 to TRAF6. What's more, TAB2 can act as a sensor for inflammatory signals by serving as a molecular beacon for recruitment of MEKK1 (MAP3K1), which in turn mediated dismissal of the NCOR/HDAC complex and permitted derepression of AR and estrogen receptor target genes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TAB2 using anti-TAB2 antibody (A02499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB2 antigen affinity purified polyclonal antibody (Catalog # A02499-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB2 at approximately 76KD. The expected band size for TAB2 is at 76KD.

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Figure 2. IHC analysis of TAB2 using anti-TAB2 antibody (A02499-1).
TAB2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TAB2 Antibody (A02499-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of TAB2 using anti-TAB2 antibody (A02499-1).
TAB2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TAB2 Antibody (A02499-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of U87 cells using anti-TAB2 antibody (A02499-1).
Overlay histogram showing U87 cells stained with A02499-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAB2 Antibody (A02499-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For TAB2 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ21885; KIAA0733CHTD2; MAP3K7IP2mitogen-activated protein kinase kinase kinase 7 interacting protein 2; Mitogen-activated protein kinase kinase kinase 7-interacting protein 2; TAB-2; TAK1-binding protein 2; TGF-beta activated kinase 1/MAP3K7 binding protein 2; TGF-beta-activated kinase 1 and MAP3K7-binding protein 2; TGF-beta-activated kinase 1-binding protein 2 TAB2 CHTD2, MAP3K7IP2, TAB-2 TGF-beta activated kinase 1 (MAP3K7) binding protein 2 TGF-beta-activated kinase 1 and MAP3K7-binding protein 2|TAK1-binding protein 2|mitogen-activated protein kinase kinase kinase 7-interacting protein 2

For more info on TAB2, check out the TAB2 Infographic

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In this infographic, you will see the following information for TAB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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