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- Table of Contents
TACC1 antibody (A04196) performs well in WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Western blot analysis of TACC1 using anti-TACC1 antibody (A04196).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse testis tissue lysate,
lane 2: U87 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACC1 antigen affinity purified polyclonal antibody (Catalog # A04196) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACC1 at approximately 100KD. The expected band size for TACC1 is at 88KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse testis tissue lysate,
lane 2: U87 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACC1 antigen affinity purified polyclonal antibody (Catalog # A04196) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACC1 at approximately 100KD. The expected band size for TACC1 is at 88KD.
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Anti-TACC1 Antibody Picoband®
TACC1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | WB |
Tested Applications
TACC1 antibody was positively detected by WB in:
mouse testis tissue, U87 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-TACC1 Antibody Picoband®
View all TACC1 Antibodies
View all TACC1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TACC1, different from the related mouse sequence by fourteen amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
87.8 kDa
Background of TACC1
Transforming acidic coiled-coil-containing protein 1 is a protein that in humans is encoded by the TACC1 gene. This locus may represent a breast cancer candidate gene. It is located close to FGFR1 on a region of chromosome 8 that is amplified in some breast cancers. Three transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-TACC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04196)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
90%
High Homology
Rabbit
80%
Moderate Homology
Dog
80%
Moderate Homology
Mouse
65%
Low homology
Rat
65%
Low homology
Cow
45%
Low homology
Sheep
45%
Low homology
Zebrafish
20%
Low homology
Drosophila
20%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TACC1 antibody
Publications
Application:
Species:
Man-jing Jiang,Shuai Huang,Li Li et al.Rubus Suavissimus Alleviates High-fat Diet-induced Lipid Metabolism Disorder via Modulation of the PPAR / SREBP Pathway in Syrian Golden Hamsters,16 December 2020,PREPRINT (Version 1) available at Research Square [ht
Species: Hamster
PMID: -
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A04196
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 21, 2026