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Product Info Summary
| SKU: | A04196 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TACC1 Antibody Picoband®
SKU/Catalog Number
A04196
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TACC1 Antibody Picoband® catalog # A04196. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TACC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04196)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TACC1, different from the related mouse sequence by fourteen amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04196 is reactive to TACC1 in Human, Mouse
Observed Molecular Weight
100 kDa
Calculated molecular weight
87.8 kDa
Background of TACC1
Transforming acidic coiled-coil-containing protein 1 is a protein that in humans is encoded by the TACC1 gene. This locus may represent a breast cancer candidate gene. It is located close to FGFR1 on a region of chromosome 8 that is amplified in some breast cancers. Three transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04196 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: mouse testis tissue, U87 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TACC1 using anti-TACC1 antibody (A04196).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse testis tissue lysate,
lane 2: U87 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACC1 antigen affinity purified polyclonal antibody (Catalog # A04196) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACC1 at approximately 100KD. The expected band size for TACC1 is at 88KD.
Specific Publications For Anti-TACC1 Antibody Picoband® (A04196)
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1 Customer Q&As for Anti-TACC1 Antibody Picoband®
Question
We are currently using anti-TACC1 antibody A04196 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The anti-TACC1 antibody (A04196) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-22
