Anti-Neurokinin 1 Receptor/TACR1 Antibody

Product Name

Anti-Neurokinin 1 Receptor/TACR1 Antibody

View all NK1R Antibodies

SKU/Catalog Number

PA1684

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neurokinin 1 Receptor/TACR1 Antibody catalog # PA1684. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neurokinin 1 Receptor/TACR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1684)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TACR1, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1684 is reactive to TACR1 in Human, Mouse, Rat

Applications

PA1684 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

46.251kDa

Background of NK1R

The tachykinin receptor 1 (TACR1) also known as neurokinin 1 receptor (NK1R) or substance P receptor (SPR) is a G protein coupled receptor found in the central nervous system and peripheral nervous system. The endogenous ligand for this receptor is Substance P, although it has some affinity for other tachykinins. The protein is the product of the TACR1 gene. Tachykinin receptor 1 consists of 407 amino acid residues, and it has a molecular weight of 58.000. Tachykinin receptor 1, as well as the other tachykinin receptors, is made of seven hydrophobic transmembrane (TM) domains with three extracellular and three intracellular loops, an amino-terminus and a cytoplasmic carboxy-terminus. The loops have functional sites, including two cysteines amino acids for a disulfide bridge, Asp-Arg-Tyr, which is responsible for association with arrestin and, Lys/Arg-Lys/Arg-X-X-Lys/Arg, which interacts with G-proteins.The tachykinin receptor 1 can be found in both the central and peripheral nervous system. It is present in neurons, brainstem, vascular endothelial cells, muscle, gastrointestinal tracts, genitourinary tract, pulmonary tissue, thyroid gland and different types of immune cells. The binding of SP to the tachykinin receptor 1 has been associated with the transmission of stress signals and pain, the contraction of smooth muscles and inflammation. Tachykinin receptor 1 antagonists have also been studied in migraine, emesis and psychiatric disorders. In fact, aprepitant has been proved effective in a number of pathophysiological models of anxiety and depression. Other diseases in which the tachykinin receptor 1 system is involved include asthma, rheumatoid arthritis and gastrointestinal disorders.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1.Western blot analysis of TACR1 using anti-TACR1antibody (PA1684).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 Whole Cell Lysate,
Lane 2: human U87 Whole Cell Lysate,
Lane 3: human COLO320 Whole Cell Lysate,
Lane 4: human SGC-7901 Whole Cell Lysate,
Lane 5: human PANC-1 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PA1684) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55KD. The expected band size for TACR1 is at 45KD

Gene/Protein Information For TACR1 (Source: Uniprot.org, NCBI)

Alternative Names

NK1R; NK-1R; NK1Rneurokinin 1 receptor; NKIR; SPR; SPRsubstance-P receptor; TAC1R; TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor); tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor); tachykinin receptor 1NK-1 receptor; TACR1 TACR1 NK1R, NKIR, SPR, TAC1R tachykinin receptor 1 substance-P receptor|NK-1 receptor|NK-1R|tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)

For more info on TACR1, check out the TACR1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TACR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the TACR1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis