Anti-TAF3 Antibody

TAF3 antibody

Boster Bio Anti-TAF3 Antibody (Catalog # A07129). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07129
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-TAF3 Antibody

View all TAF3 Antibodies

SKU/Catalog Number

A07129

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TAF3 Antibody (Catalog # A07129). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TAF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07129)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TAF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07129 is reactive to TAF3 in Human, Mouse, Rat

Applications

A07129 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

103.582kDa

Background of TAF3

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Gangloff Y.G., Mol. Cell. Biol. 21:5109-5121(2001) [PubMed: 11438666].
Deloukas P., Nature 429:375-381(2004) [PubMed: 15164054].
Bechtel S., BMC Genomics 8:399-399(2007) [PubMed: 17974005].

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For TAF3 (Source: Uniprot.org, NCBI)

Gene Name

TAF3

Full Name

Transcription initiation factor TFIID subunit 3

Weight

103.582kDa

Superfamily

TAF3 family

Alternative Names

140 kDa TATA box-binding protein-associated factor; MGC25063; RNA polymerase II transcription factor TAFII140; TAF(II)140; TAF140; TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa; TAFII140; TAFII-140; TBP-associated factor 3; transcription initiation factor TFIID 140 kDa subunit TAF3 TAF140, TAFII-140, TAFII140 TATA-box binding protein associated factor 3 transcription initiation factor TFIID subunit 3|140 kDa TATA box-binding protein-associated factor|RNA polymerase II transcription factor TAFII140|TAF(II)140|TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa|TBP-associated factor 3|transcription initiation factor TFIID 140 kDa subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TAF3, check out the TAF3 Infographic

TAF3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TAF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-TAF3 Antibody

Question

We are currently using anti-TAF3 antibody A07129 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-21

Answer

The anti-TAF3 antibody (A07129) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-21

Question

I was wanting to use your anti-TAF3 antibody for WB for rat bone on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat bone identification?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

As indicated on the product datasheet, A07129 anti-TAF3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-21

Question

Here is the WB image, lot number and protocol we used for bone using anti-TAF3 antibody A07129. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-11

Question

Do you have a BSA free version of anti-TAF3 antibody A07129 available?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TAF3 antibody A07129 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-20

Question

My lab would like to test anti-TAF3 antibody A07129 on rat bone for research purposes, then I may be interested in using anti-TAF3 antibody A07129 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

The products we sell, including anti-TAF3 antibody A07129, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-23

Order DetailsPrice
A07129-100uL

100uL

$360

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A07129
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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