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Product Info Summary
| SKU: | A05211 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone chaperone ASF1B ASF1B Antibody
SKU/Catalog Number
A05211
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Histone chaperone ASF1B ASF1B Antibody (Catalog# A05211). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone chaperone ASF1B ASF1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05211)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ASF1B. Immunogen sequence location: 101-150
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05211 is reactive to ASF1B in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
22.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05211 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:5000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of lysates from K562 cells, using ASF1B Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Histone chaperone ASF1B ASF1B Antibody (A05211)
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Customer Q&As
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5 Customer Q&As for Anti-Histone chaperone ASF1B ASF1B Antibody
Question
Would A05211 anti-ASF1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Jha
Verified customer
Asked: 2020-01-15
Answer
You can see on the product datasheet, A05211 anti-ASF1B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-15
Question
Is a blocking peptide available for product anti-ASF1B antibody (A05211)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
We do provide the blocking peptide for product anti-ASF1B antibody (A05211). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-21
Question
We are currently using anti-ASF1B antibody A05211 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
The anti-ASF1B antibody (A05211) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-28
Question
Please see the WB image, lot number and protocol we used for teratocarcinoma using anti-ASF1B antibody A05211. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-26
Question
Can you help my question with product A05211, anti-ASF1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Zhao
Verified customer
Asked: 2016-04-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05211 anti-ASF1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-04-04
