Anti-Histone chaperone ASF1B ASF1B Antibody

ASF1B antibody

Boster Bio Anti-Histone chaperone ASF1B ASF1B Antibody (Catalog# A05211). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05211
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

Product Name

Anti-Histone chaperone ASF1B ASF1B Antibody

View all ASF1B Antibodies

SKU/Catalog Number

A05211

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Histone chaperone ASF1B ASF1B Antibody (Catalog# A05211). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone chaperone ASF1B ASF1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05211)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human ASF1B. Immunogen sequence location: 101-150

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05211 is reactive to ASF1B in Human, Mouse, Rat

Applications

A05211 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

22.434kDa

Background of ASF1B

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For ASF1B (Source: Uniprot.org, NCBI)

Gene Name

ASF1B

Full Name

Histone chaperone ASF1B

Weight

22.434kDa

Superfamily

ASF1 family

Alternative Names

ASF1 silencing function 1 homolog B (S. cerevisiae); CCG1-interacting factor A-II; CIA-II; FLJ10604; hAsf1; hAsf1b; hCIA-II; histone chaperone ASF1B; silencing function protein 1 homolog B ASF1B CIA-II anti-silencing function 1B histone chaperone histone chaperone ASF1B|ASF1 anti-silencing function 1 homolog B|CCG1-interacting factor A-II|anti-silencing function protein 1 homolog B|hAsf1|hAsf1b|hCIA-II

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ASF1B, check out the ASF1B Infographic

ASF1B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ASF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Histone chaperone ASF1B ASF1B Antibody

Question

Would A05211 anti-ASF1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

W. Jha

Verified customer

Asked: 2020-01-15

Answer

You can see on the product datasheet, A05211 anti-ASF1B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-15

Question

Is a blocking peptide available for product anti-ASF1B antibody (A05211)?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

We do provide the blocking peptide for product anti-ASF1B antibody (A05211). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-21

Question

We are currently using anti-ASF1B antibody A05211 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2017-08-28

Answer

The anti-ASF1B antibody (A05211) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-08-28

Question

Please see the WB image, lot number and protocol we used for teratocarcinoma using anti-ASF1B antibody A05211. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-26

Question

Can you help my question with product A05211, anti-ASF1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Zhao

Verified customer

Asked: 2016-04-04

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05211 anti-ASF1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-04-04

Order DetailsPrice
A05211

100 μL

$335

Free Secondary Antibody

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A05211
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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