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Product Info Summary
| SKU: | A05993-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, ICC, WB |
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Product info
Product Name
Anti-TAF6 Antibody Picoband®
SKU/Catalog Number
A05993-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAF6 Antibody Picoband® catalog # A05993-1. Tested in WB, ICC/IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TAF6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05993-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human TAF6 recombinant protein (Position: S16-R479). Human TAF6 shares 98.9% amino acid (aa) sequence identity with both mouse and rat TAF6.
Reactive Species
A05993-1 is reactive to TAF6 in Human, Mouse, Rat
Observed Molecular Weight
73 kDa
Calculated molecular weight
72.7 kDa
Background of TAF6
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05993-1 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of TAF6 using anti-TAF6 antibody (A05993-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF6 antigen affinity purified polyclonal antibody (A05993-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAF6 at approximately 73 kDa. The expected band size for TAF6 is at 73 kDa.
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IF analysis of TAF6 using anti-TAF6 antibody (A05993-1) and anti-Beta Tubulin antibody (M01857-3).
TAF6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TAF6 Antibody (A05993-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunoprecipitating TAF6 in MCF-7 whole cell lysate.
Western blot analysis of TAF6 using anti-TAF6 antibody (A05993-1).
Lane 1: MCF-7 whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-TAF6 antibody in MCF-7 whole cell lysate,
Lane 3: anti-TAF6 antibody (2μg) + MCF-7 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TAF6 antigen affinity purified polyclonal antibody (A05993-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TAF6 at approximately 73 kDa. The expected band size for TAF6 is at 73 kDa.
Specific Publications For Anti-TAF6 Antibody Picoband® (A05993-1)
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