Anti-TAF9 Antibody

Product Name

Anti-TAF9 Antibody

View all TAF9 Antibodies

SKU/Catalog Number

A05319

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TAF9 Antibody (Catalog # A05319). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TAF9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-TAF9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05319)

Host

Rabbit

Contents

TAF9 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

TAF9 antibody was raised against a 17 amino acid peptide near the center of human TAF9. The immunogen is located within amino acids 110 - 160 of TAF9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members.

Reactive Species

A05319 is reactive to TAF9 in Human, Mouse, Rat

Applications

A05319 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

28.974kDa

Background of TAF9

Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TAF9 in HepG2 cell lysate with TAF9 antibody at (A) 1 and (B) 2μg/ml.

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Immunohistochemistry of TAF9 in mouse liver tissue with TAF9 antibody at 5 μg/mL.

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Immunofluorescence of TAF9 in mouse liver tissue with TAF9 antibody at 20 μg/mL.

Gene/Protein Information For TAF9 (Source: Uniprot.org, NCBI)

Alternative Names

AD-004; adenylate kinase 6; adenylate kinase isoenzyme 6; adrenal gland protein AD-004; AK6CIP; ATP-AMP transphosphorylase 6; CGI-137; CINAPMGC:1603; coilin interacting protein; Coilin-interacting nuclear ATPase protein; EC 2.7.4.3; hCINAP; MGC1603; MGC3647; MGC5067; RNA polymerase II TBP-associated factor subunit G; STAF31/32; TAF2GMGC:5067; TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa; TAFII-31; TAFII31MGC:3647; TAFII-32; TAFII32TATA box binding protein (TBP)-associated factor, RNA polymerase II, G, 32kD; TAFIID32; transcription initiation factor TFIID 31 k TAF9 MGC:5067, STAF31/32, TAF2G, TAFII-31, TAFII-32, TAFII31, TAFII32, TAFIID32 TATA-box binding protein associated factor 9 transcription initiation factor TFIID subunit 9|RNA polymerase II TBP-associated factor subunit G|TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa|transcription initiation factor TFIID 31 kD subunit|transcription initiation factor TFIID 31 kDa subunit|transcription initiation factor TFIID 32 kDa subunit

For more info on TAF9, check out the TAF9 Infographic

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In this infographic, you will see the following information for TAF9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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