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Product Info Summary
| SKU: | A07896-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TAS1R1 Antibody Picoband®
SKU/Catalog Number
A07896-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAS1R1 Antibody Picoband® catalog # A07896-1. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TAS1R1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07896-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse TAS1R1 recombinant protein (Position: E102-T842). Mouse TAS1R1 shares 72.9% and 88.8% amino acid (aa) sequence identity with human and rat TAS1R1, respectively.
Reactive Species
A07896-1 is reactive to TAS1R1 in Mouse, Rat
Observed Molecular Weight
140 kDa
Calculated molecular weight
93.4 kDa
Background of TAS1R1
Predicted to enable G protein-coupled receptor activity and taste receptor activity. Predicted to be involved in sensory perception of umami taste. Predicted to be active in plasma membrane. Is expressed in tongue anterior part. Orthologous to human TAS1R1 (taste 1 receptor member 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07896-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of TAS1R1 using anti-TAS1R1 antibody (A07896-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS1R1 antigen affinity purified polyclonal antibody (A07896-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAS1R1 at approximately 140 kDa. The expected band size for TAS1R1 is at 93 kDa.
Specific Publications For Anti-TAS1R1 Antibody Picoband® (A07896-1)
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