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Product Info Summary
| SKU: | A08729-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TAS1R2 Antibody Picoband®
SKU/Catalog Number
A08729-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAS1R2 Antibody Picoband® catalog # A08729-2. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TAS1R2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08729-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human TAS1R2 recombinant protein (Position: Y96-D839). Human TAS1R2 shares 69.6% and 71% amino acid (aa) sequence identity with mouse and rat TAS1R2, respectively.
Reactive Species
A08729-2 is reactive to TAS1R2 in Human
Observed Molecular Weight
100 kDa
Calculated molecular weight
95.2 kDa
Background of TAS1R2
T1R2 - Taste receptor type 1 member 2 is a protein that in humans is encoded by the TAS1R2 gene. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08729-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PANC-1 whole cell, human Caco-2 whole cell, human 293T whole cell, human COLO 320 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TAS1R2 using anti-TAS1R2 antibody (A08729-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human COLO 320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS1R2 antigen affinity purified polyclonal antibody (Catalog # A08729-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAS1R2 at approximately 100 kDa. The expected band size for TAS1R2 is at 95 kDa.
Specific Publications For Anti-TAS1R2 Antibody Picoband® (A08729-2)
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