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Product Info Summary
| SKU: | A10959 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-TAS2R10 Antibody
SKU/Catalog Number
A10959
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TAS2R10 Antibody (Catalog# A10959). Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAS2R10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10959)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TAS2R10. Immunogen sequence location: 122 - 171
Reactive Species
A10959 is reactive to TAS2R10 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
35.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10959 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of the lysates from HepG2 cells using TAS2R10 antibody.
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Immunofluorescence analysis of MCF7 cells, using TAS2R10 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO cells, using TAS2R10 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TAS2R10 Antibody (A10959)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TAS2R10 Antibody
Question
We are currently using anti-TAS2R10 antibody A10959 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
The anti-TAS2R10 antibody (A10959) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-01
Question
Is this A10959 anti-TAS2R10 antibody reactive to the isotypes of TAS2R10?
P. Jones
Verified customer
Asked: 2017-12-20
Answer
The immunogen of A10959 anti-TAS2R10 antibody is A peptide derived from human TAS2R10. Immunogen sequence location: 122 - 171. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-20
Question
I was wanting to use your anti-TAS2R10 antibody for WB for human intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human intestine identification?
G. Mangal
Verified customer
Asked: 2015-08-27
Answer
It shows on the product datasheet, A10959 anti-TAS2R10 antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-27
Question
I would like to test anti-TAS2R10 antibody A10959 on human intestine for research purposes, then I may be interested in using anti-TAS2R10 antibody A10959 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Anderson
Verified customer
Asked: 2013-04-01
Answer
The products we sell, including anti-TAS2R10 antibody A10959, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-04-01
