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Product Info Summary
| SKU: | M05588 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-TAX1BP3 Monoclonal Antibody
SKU/Catalog Number
M05588
BM5398 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TAX1BP3 Monoclonal Antibody catalog # M05588. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAX1BP3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05588)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADDC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TAX1BP3 May play a role in the Rho signaling pathway. May act as an inhibitor of the Wnt signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6.
Reactive Species
M05588 is reactive to TAX1BP3 in Human, Mouse, Rat
Observed Molecular Weight
14 kDa
Calculated molecular weight
13.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05588 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:20
Positive Control
WB: human HT1080 whole cell, human SIHA whole cell, human A549 whole cell, human Hacat whole cell, rat small intestine tissue, rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TAX1BP3 using anti-TAX1BP3 antibody (M05588).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT1080 whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat small intestine tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAX1BP3 antigen affinity purified monoclonal antibody (M05588) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAX1BP3 at approximately 14 kDa. The expected band size for TAX1BP3 is at 14 kDa.
Specific Publications For Anti-TAX1BP3 Monoclonal Antibody (M05588)
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7 Customer Q&As for Anti-TAX1BP3 Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for kidney {eco:0000312 using anti-TAX1BP3 Monoclonal antibody M05588. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
I was wanting to use your anti-TAX1BP3 Monoclonal antibody for WB for human kidney eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney {eco:0000312 identification?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
It shows on the product datasheet, M05588 anti-TAX1BP3 Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
We want to test anti-TAX1BP3 Monoclonal antibody M05588 on human kidney {eco:0000312 for research purposes, then I may be interested in using anti-TAX1BP3 Monoclonal antibody M05588 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The products we sell, including anti-TAX1BP3 Monoclonal antibody M05588, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-14
Question
Does anti-TAX1BP3 Monoclonal antibody M05588 work for WB with kidney {eco:0000312?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
According to the expression profile of kidney {eco:0000312, TAX1BP3 is highly expressed in kidney {eco:0000312. So, it is likely that anti-TAX1BP3 Monoclonal antibody M05588 will work for WB with kidney {eco:0000312.
Boster Scientific Support
Answered: 2019-04-12
Question
We are currently using anti-TAX1BP3 Monoclonal antibody M05588 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
G. Lewis
Verified customer
Asked: 2017-11-24
Answer
The anti-TAX1BP3 Monoclonal antibody (M05588) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-24
Question
Will anti-TAX1BP3 Monoclonal antibody M05588 work on goat WB with brain {eco:0000312?
T. Li
Verified customer
Asked: 2016-11-02
Answer
Our lab technicians have not tested anti-TAX1BP3 Monoclonal antibody M05588 on goat. You can run a BLAST between goat and the immunogen sequence of anti-TAX1BP3 Monoclonal antibody M05588 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain {eco:0000312 in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-11-02
Question
Is this M05588 anti-TAX1BP3 Monoclonal antibody reactive to the isotypes of TAX1BP3?
J. Roberts
Verified customer
Asked: 2013-08-29
Answer
The immunogen of M05588 anti-TAX1BP3 Monoclonal antibody is A synthesized peptide derived from human TAX1BP3 May play a role in the Rho signaling pathway. May act as an inhibitor of the Wnt signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-08-29
